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KMID : 0950619990080010059
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Journanl of Allied Health Sciences
1999 Volume.8 No. 1 p.59 ~ p.66
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Effect of Estrogen on the Apoptosis in Osteoblast cells
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Jung Soo-Kyung
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Abstract
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Apoptosis is a naturally occurring cell suicide pathway induced in osteoblast. The absence of a survival factor, such as particular hormone or growth factor, will induce a cell to initiate its own cell death. This process is thought to play a role in the pathophysiology of postmenopausal osteoporosis. Estrogen exerts direct effects on bone cells, but poorly understood its role in the maintenance of cell survival and apoptosis. Based on the importance of survival factors and cytokines in bone remodeling, some studies suggest that estrogen acts on bone cell by modulating the cytokine production that increases bone resorption. However other studies have not investigated the effect of estrogen on the apoptosis of osteoblast cells. To understand how estrogens regulate the apoptosis of osteoblast cells, the physiologically active etrogen metabolite 17 beta-estradiol was tested to determine its effects on the well characterized murine osteoblastic cell-line MC3T3-E1. Experiments were designed to identify the effects of estrogen on TNF-α-induced apoptosis. Murine osteoblastic MC3T3-EI cells underwent apoptosis following removal of serum, or addition of TNF-α(10^(-10)) as indicated by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling. Estrogen inhibited the apoptosis of osteoblast cells induced by 10^(-10)M TNF-α. This in vitro evidence suggests that estrogen might exert at least part of antiapoptotic influence on the bone cells.
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KEYWORD
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